Journal: bioRxiv
Article Title: Identification of a Sulf2-dependant astrocyte subtype that stands out through the expression of Olig2 in the ventral spinal cord
doi: 10.1101/430074
Figure Lengend Snippet: All images show transverse spinal cord sections. A, B : Combined detection of Olig2 (green), PDGFRα (red) and Sox10 (A, blue) or Olig1 (B, blue) viewed on E13.5 hemi-ventral spinal cord. Note the presence of Olig2+ cells that are not stained with the OPC markers (arrows). C-F : Combined detection of Olig2 (green) and mRNAs (red) encoding for fgfr3 (C, D), aldh1L1 (E) and tenascin-C (F) viewed on E13.5 (C) and E15.5 (D-F) hemi-ventral spinal cords. Note, in all panels, the presence of Olig2+ cells stained with mRNA probes (arrows). G-G’’ : Combined detection of Olig2 (blue), Sox10 (red) and GFP (green) on E13.5 Aldh1L1-GFP transgenic embryo. Images show high magnification of the ventral progenitor zone. Horizontal sets present successively Olig2 staining, Sox10 staining, and the merged image. Note the presence of GFP+/Olig2+/Sox10 - cells (arrows) both in the progenitor zone (Olig2+ pMN domain) and in the adjacent spinal parenchyma. H-h’’ : Combined detection of Olig2 (blue), Sox10 (red) and GFP (green) on E16.5 Aldh1L1-GFP transgenic embryo. h, h’ and h’’ show higher magnifications of the area framed in H, H’ and H’’, respectively. Arrows point to GFP+/Olig2+/Sox10 - cells. Scale bars = 100 μm in H-H’’, 50 μm in A-F, h-h’’ and 25 μm in G-G’’. See also Figures S2.
Article Snippet: Antibodies used in this study were as follows: goat anti-AldoC (SCB), rabbit and mouse anti-GFAP (DAKO), rabbit anti-NFIA (Active Motif), rabbit and goat anti-Olig2 (Millipore and R&D Systems), goat anti-Olig1 (R&D Systems), goat anti-Sox10 (Santa Cruz Biotechnology), rat anti-PDGFRα (BD Pharmingen), rabbit anti-Zeb1 (Novus) and mouse anti-Nkx6.1 (Hybridoma Bank).
Techniques: Staining, Transgenic Assay
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